Genome stability is essential for cell viability and prevention of malignant transformation. We study mechanisms by which genome integrity is threatened and maintained in both healthy and malignant cells. The genome is under constant onslaught from endogenous and exogenous sources of DNA damage which can threaten genome integrity resulting in either cell death or malignant transformation. We are interested in the etiology of DNA damage and DNA repair processes. In particular, we are interested in the SMC5/6 complex, a chromosomal maintenance complex similar to condensin and cohesion, but with a more enigmatic function. We are using molecular, biochemical, and cell culture strategies to investigate how SMC5/6 impacts genome integrity.